Bacterial communities in the rumen and feces of lactating Holstein dairy cows are not affected when fed reduced-fat dried distillers’ grains with solubles

Reduced-fat dried distillers’ grains with solubles (RF-DDGSs) are co-products of ethanol production and contain less fat than traditional grains. The in corn is ~91% unsaturated, it toxic to rumen microorganisms so could influence the composition microbiome. It has been demonstrated that RF-DDGS a suitable ration ingredient support high-producing dairy cow, this feedstuff promising alternative protein source for lactating cows. current study aims better understand effect on fecal bacterial Thirty-six multiparous (two or three), mid-lactation Holstein cows (BW = 680 ± 11 kg; 106 27 DIM) were randomly assigned two groups which fed control diet made up corn, silage, alfalfa hay supplemented expeller soybean meal added (20% DM) containing approximately 6.0% fat. Whole contents (rumen fluid digesta; esophageal tubing method) feces (free-catch collected day 35 experimental period, after 14-d acclimation period. Rumen from each cow used DNA extraction. community samples was assessed via 16S rRNA gene by using Illumina MiSeq sequencing platform. Bacteroidetes, Actinobacteria, Firmicutes most abundant phyla contents. microbiota dominated as well Actinobacteria Chloroflexi. RF-DGGS increased richness, evenness, Shannon diversity both associated several taxa had different abundance treatment versus comparisons. RF-DGGS, however, did not significantly alter feces. In general, these findings dietary inclusion impose any serious short-term (within 30 days) health consequences, would be expected. With study, we present further evidence 20% (DM basis) can done without consequence microbiome rumen.